Nobuhiro Morone

Biography:

2015-Present Senior Investigator Scientist at MRC Toxicology Unit and visiting Professor at Kyoto University, Kyoto, Japan.

2010-2015 Senior Lecturer at iCeMS Kyoto University, Kyoto, Japan.

2005-2010 Section Chief at National Institute of Neuroscience, National Centre of Neurology and Psychiatry, Tokyo, Japan.

2004-2005 PI at Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan.

1999-2003 Postdoctoral at ERATO-JST/Nagoya University, Nagoya, Japan.

1999 PhD (Kyoto University, Japan) Synthetic & Biological Chemistry.

Research Interests:

Nobu’s research in the Unit is based on “EM-based nano-toxicology” for visual understanding the role of endocytosis at the plasma membrane and membrane recycling among the intracellular organelle compartments affected by toxins or drugs for mesothelioma in immune-oncology and neurogenerative diseases as well. Nobu’s EM covers a whole range of cellular tissues from the mouse brain to the signalling receptor-complexes by using both classic and advanced microscopy from deep-etch EM and cryo-EM (single particle analysis and tomography). Nobu’s future goal is to determine the atomic structure of crucial membrane machinery (caveolae with MSK) and chromatin complex in situ by the hybrid EM correlation, as well as to establish a lesion detection system under EM resolution by a template matching method toward tumour and neurogenerative diseases.

UK-Japan Structural Life Science Forum (Chair)

frontiers_nobu3

Publications

Key publications:

1. Io S, Kabata M, Iemura Y, Semi K, Morone N, Minagawa A, Wang B, Okamoto I, Nakamura T, Kojima Y, Iwatani C, Tsuchiya H, Kaswandy B, Kondoh E, Kaneko S, Woltjen K, Saitou M, Yamamoto T, Mandai M, Takashima Y. (2021) Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell. 28(6):1023-1039.e13.

2. Fox JL, Hughes MA, Meng X, Sarnowska NA, Powley IR, Jukes-Jones R, Dinsdale D, Ragan TJ, Fairall L, Schwabe JWR, Morone N, Cain K, MacFarlane M. (2021) Cryo-EM structural analysis of FADD:Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate. Nat Commun. 12(1):819.

3. Amelio I, Panatta E, Niklison-Chirou MV, Steinert JR, Agostini M, Morone N, Knight RA, Melino G. (2020) The C terminus of p73 is essential for hippocampal development. Proc Natl Acad Sci USA. 117(27):15694-15701.

4. Song Y, Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A, Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR. (2020) Mechanism of crosstalk between the LSD1 demethylase and HDAC1 deacetylase in the CoREST complex. Cell Rep. 30(8):2699-2711.e8.

5. Robinson SW, Bourgognon JM, Spiers JG, Breda C, Campesan S, Butcher A, Mallucci GR, Dinsdale D, Morone N, Mistry R, Smith TM, Guerra-Martin M, Challiss RAJ, Giorgini F, Steinert JR. (2018) Nitric oxide-mediated posttranslational modifications control neurotransmitter release by modulating complexin farnesylation and enhancing its clamping ability. PLoS Biol. 16(4):e2003611. doi: 10.1371/journal.pbio.2003611.

6. Nishimura T, Morone N, Suetsugu S. (2018) Membrane re-modelling by BAR domain superfamily proteins via molecular and non-molecular factors. Biochem Soc Trans. 46(2):379-389. doi: 10.1042/BST20170322.

7. Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T. (2017) Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency. Cell Metab. 25(5):1103-1117. doi: 10.1016/j.cmet.2017.04.017.

8. Fujiwara TK, Iwasawa K, Kalay Z, Tsunoyama TA, Watanabe Y, Umemura YM, Murakoshi H, Suzuki KG, Nemoto YL, Morone N, Kusumi A. (2016) Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane. Mol Biol Cell. 27(7):1101-19. doi: 10.1091/mbc.E15-04-0186.

9. Kadota S, Minami I, Morone N, Heuser JE, Agladze K, Nakatsuji N. (2013) Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets. Eur Heart J. 34(15):1147-56.

10. Kusumi A, Fujiwara TK, Morone N, Yoshida KJ, Chadda R, Xie M, Kasai RS, Suzuki KG. (2012) Membrane mechanisms for signal transduction: the coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes. Semin Cell Dev Biol. 23(2):126-44.

11. Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N. (2012) A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2(5):1448-60.

12. Sinha B, Köster D, Ruez R, Gonnord P, Bastiani M, Abankwa D, Stan RV, Butler-Browne G, Vedie B, Johannes L, Morone N, Parton RG, Raposo G, Sens P, Lamaze C, Nassoy P. (2011) Cells respond to mechanical stress by rapid disassembly of caveolae. Cell. 144(3):402-13.

Other publications:

13. Morone N, Nakada C, Umemura Y, Usukura J, Kusumi A. (2008) Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography. Methods Cell Biol. 88: 207-36.

14. Yao I, Takagi H, Ageta H, Kahyo T, Sato S, Hatanaka K, Fukuda Y, Chiba T, Morone N, Yuasa S, Inokuchi K, Ohtsuka T, Macgregor GR, Tanaka K, Setou M. (2007) SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Cell. 130:943-57.

15. Morone N, Fujiwara T, Murase K, Kasai SR, Ike H, Yuasa S, Usukura J, Kusumi A. (2006) Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. J Cell Biol. 174:851-62.

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