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1) FEI AMIRA for SEM or TEM-based 3D reconstruction.

2) FEI Inspect 3D for tomography.

3) IMOD for tomography.

4) EMAN2 & RELION for single-particle analysis & 3D model.




1) Wacom Cintiq 27 QHD touch.




Worflow (AMIRA):

1) Import the serial image data with specific voxel size.

2) Image filtering for noise reduction.

3) 3D exploration.

4) Semi-manual segmentation.

5) Surface reconstruction for label fields.

6) Measurement and analysis for organelle volume.