CEMOVIS

Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) for nanoscale molecular structures.

Workflow:

1. Vitrification tissue cells by Leica EM HPM100, TFS Vitrobot, or plunge freezing in super cooled slush nitrogen
2. Set onto the specimen pins for cryo-ultramicrotomy
3. Cryo-trim the frozen block with Diatome cryo-trim diamond knife (35°) by Leica EM UC7-FC7 at liquid nitrogen temperature
4. Cryo-ultra-section with Diatome cryo-immune diamond knife (35°) and making ribbons by Leica EM Crion
5. Collection the frozen ribbon on the grid by Leica EM double-manipulator
6. Transfer the cryo-grids to the cryo-storage box
7. Set into the cryo-transfer holder
8. General alignments for cryo-EM
9. Low-dose imaging by cryo-EM and tomography
10. Analysis for nanoscale molecular structures