skip to content
 

 

Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) for nanoscale molecular structures.

 

Workflow:

  1. Vitrification tissue cells by Leica EM HPM100, TFS Vitrobot, or plunge freezing in super cooled slush nitrogen
  2. Set onto the specimen pins for cryo-ultramicrotomy
  3. Cryo-trim the frozen block with Diatome cryo-trim diamond knife (35°) by Leica EM UC7-FC7 at liquid nitrogen temperature
  4. Cryo-ultra-section with Diatome cryo-immune diamond knife (35°) and making ribbons by Leica EM Crion
  5. Collection the frozen ribbon on the grid by Leica EM double-manipulator
  6. Transfer the cryo-grids to the cryo-storage box
  7. Set into the cryo-transfer holder
  8. General alignments for cryo-EM
  9. Low-dose imaging by cryo-EM and tomography
  10. Analysis for nanoscale molecular structures

Group Members

Jaime Llodra Gonzalez

EM Research Specialist

jl2235@mrc-tox.cam.ac.uk

 

Maria Guerra Martin

Senior Research Officer

mag88@mrc-tox.cam.ac.uk

 

Nobuhiro Morone

Senior Investigator Scientist

Facility Head

nm669@mrc-tox.cam.ac.uk