skip to content


Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) for nanoscale molecular structures.



  1. Vitrification tissue cells by Leica EM HPM100, TFS Vitrobot, or plunge freezing in super cooled slush nitrogen
  2. Set onto the specimen pins for cryo-ultramicrotomy
  3. Cryo-trim the frozen block with Diatome cryo-trim diamond knife (35°) by Leica EM UC7-FC7 at liquid nitrogen temperature
  4. Cryo-ultra-section with Diatome cryo-immune diamond knife (35°) and making ribbons by Leica EM Crion
  5. Collection the frozen ribbon on the grid by Leica EM double-manipulator
  6. Transfer the cryo-grids to the cryo-storage box
  7. Set into the cryo-transfer holder
  8. General alignments for cryo-EM
  9. Low-dose imaging by cryo-EM and tomography
  10. Analysis for nanoscale molecular structures