Negative staining for purified protein complexes.
Workflow:
- Glow-discharge the carbon membrane/ copper grid by the Quorum GloQube
- Cross-link the purified protein-complexes with glutaraldehyde (as required)
- Quench with Tris buffer (as required)
- Exchange the buffer with the spin column (as required)
- Put the complex (0.1-1.0 µg/µl x 5 µl) on the carbon membrane/ copper grid
- Interact in fridge overnight (as required)
- Handle with anti-capillary crossover tweezers
- Stain the grid with uranyl acetate (or UA-Non alternative) for 60 sec
- Blot with tiny pieces of filter papers
- Natural dry on the filter paper
- TEM imaging
- 2D classification (as required)
