
Tissue Selection - Fixed tissue samples are selected and trimmed/dissected if needed and placed into printed cassettes for tissue processing. |
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Tissue Processing - The process of removing water from tissue samples and infiltrating them with a rigid substance is essential for high quality tissue sections. |
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Embedding
- Processed tissues are oriented in moulds filled with paraffin wax to create an FFPE tissue block.
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Microtomy
- Sections are prepared by highly skilled staff using automatic rotary microtomes.
- Thin tissue sections are mounted on glass slides for further investigation. |
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Haematoxylin and Eosin Staining - H&E is the most commonly used histological stain, haematoxylin stains the nuclei blue and eosin stains cytoplasm and connective tissue red/pink demonstrating good intra cellular detail for a huge range of cell types and structures. |