Cell analysers
The Flow Cytometry Facility has three analytical cytometers including one with plate-reading.
Examples of application:
- Immunophenotyping
- Cell cycle
- Proliferation assays
- Apoptosis and cell viability assays
Instruments available:
- BD Fortessa LSR (5 lasers, can detect up to 18 colours at once)
- BD Fortessa LSR with high-throughput sampler (4 lasers, can detect up to 16 colours at once) – able to take 96-well plates for analysis of cells in suspension and acquire 40,000 events per second
- BD FACS Canto II (2 lasers, can detect up to 8 fluorescent signals)
The analysers are user-operated but all users require two training sessions (the second with user samples) before they can be used independently. After training, users requiring assistance or wishing to further their analyses in software such as FlowJo or FCSExpress may contact the facility.
Enquiries relating to training and use of the cytometers should be directed to cytometry@mrc-tox.cam.ac.uk.