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MRC Toxicology Unit

 

Biography

2015-Present Senior Investigator Scientist at MRC Toxicology Unit and visiting Professor at Kyoto University, Kyoto, Japan.

2010-2015 Senior Lecturer at iCeMS Kyoto University, Kyoto, Japan.

2005-2010 Section Chief at National Institute of Neuroscience, National Centre of Neurology and Psychiatry, Tokyo, Japan.

2004-2005 PI at Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan.

1999-2003 Postdoctoral at ERATO-JST/Nagoya University, Nagoya, Japan.

1999 PhD (Kyoto University, Japan) Synthetic & Biological Chemistry.

Research

3D electron microscopy for molecular and cellular medicine and toxicology:

-SBF SEM (Mitochondrial dysfunction)

-Tomography (Endocytosis at plasma membrane)

-Single particle analysis (Cell death)

-CEMOVIS (Skin epidermis)

-CLEM (Neuronal muscular junction)

-Rapid-freezing technique

-Freeze fracturing & deep-etching techniques (Endocytic machinery as caveolae, clathrin-coated pits, and MSK)

Publications

Key publications: 

 

Nitric oxide-mediated posttranslational modifications control neurotransmitter release by modulating complexin farnesylation and enhancing its clamping ability.

Robinson SW, Bourgognon JM, Spiers JG, Breda C, Campesan S, Butcher A, Mallucci GR, Dinsdale D, Morone N, Mistry R, Smith TM, Guerra-Martin M, Challiss RAJ, Giorgini F, Steinert JR.

PLoS Biol. (2018) 16(4):e2003611. doi: 10.1371/journal.pbio.2003611.

 

Membrane re-modelling by BAR domain superfamily proteins via molecular and non-molecular factors.

Nishimura T, Morone N, Suetsugu S.

Biochem Soc Trans. (2018) 46(2):379-389. doi: 10.1042/BST20170322.

 

Human pluripotent stem cell-derived cardiac tissue-like constructs for repairing the infarcted myocardium.

Li J, Minami I, Shiozaki M, Yu L, Yajima S, Miyagawa S, Shiba Y, Morone N, Fukushima S, Yoshioka M, Li S, Qiao J, Li X, Wang L, Kotera H, Nakatsuji N, Sawa Y, Chen Y, Liu L.

Stem Cell Reports. (2017) 9(5):1546-1559. doi: 10.1016/j.stemcr.2017.09.007

 

Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension.

Tachikawa M, Morone N, Senju Y, Sugiura T, Hanawa-Suetsugu K, Mochizuki A, Suetsugu S.

Sci Rep. (2017) 7(1):7794. doi: 10.1038/s41598-017-08259-5.

 

Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency.

Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T.

Cell Metab. (2017) 25(5):1103-1117. doi: 10.1016/j.cmet.2017.04.017.

 

Polymer-coated pH-responsive high-density lipoproteins.

Kim H, Okamoto H, Felber AE, Polomska A, Morone N, Heuser JE, Leroux JC, Murakami T.

J Control Release. (2016) 228:132-140. doi: 10.1016/j.jconrel.2016.03.005.

 

The modeling of Alzheimer’s disease by the overexpression of mutant Presenilin 1 in human embryonic stem cells.

Honda M, Minami I, Tooi N, Morone N, Nishioka H, Uemura K, Kinoshita A, Heuser JE, Nakatsuji N, Aiba K.

Biochem Biophys Res Commun. 2016 Jan 15;469(3):587-92. doi: 10.1016/j.bbrc.2015.12.025.

 

Thermosensitive Ion Channel Activation in Single Neuronal Cells by Using Surface-Engineered Plasmonic Nanoparticles.

Nakatsuji H, Numata T, Morone N, Kaneko S, Mori Y, Imahori H, Murakami T.

Angew Chem Int Ed Engl. 2015 Sep 28;54(40):11725-9. doi: 10.1002/anie.201505534.

 

Diffusion-coupled molecular assembly: structuring of coordination polymers across multiple length scales.

Hirai K, Reboul J, Morone N, Heuser JE, Furukawa S, Kitagawa S.

J Am Chem Soc. 2014 Oct 22;136(42):14966-73. doi: 10.1021/ja507971r.

 

Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin.

Weng L, Enomoto A, Miyoshi H, Takahashi K, Asai N, Morone N, Jiang P, An J, Kato T, Kuroda K, Watanabe T, Asai M, Ishida-Takagishi M, Murakumo Y, Nakashima H, Kaibuchi K, Takahashi M.

EMBO J. 2014 Sep 17;33(18):2098-112. doi: 10.15252/embj.201488289.

 

Mesoscopic metal nanoparticles doubly functionalized with natural and engineered lipidic dispersants for therapeutics.

Murakami T, Nakatsuji H, Morone N, Heuser JE, Ishidate F, Hashida M, Imahori H.

ACS Nano. 2014 Jul 22;8(7):7370-6. doi: 10.1021/nn5024818.

 

Helical DNA origami tubular structures with various sizes and arrangements.

Endo M, Yamamoto S, Emura T, Hidaka K, Morone N, Heuser JE, Sugiyama H.

Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7484-90. doi: 10.1002/anie.201402973.

 

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging.

Nishimura H, Ritchie K, Kasai RS, Goto M, Morone N, Sugimura H, Tanaka K, Sase I, Yoshimura A, Nakano Y, Fujiwara TK, Kusumi A.

J Cell Biol. 2013 Sep 16;202(6):967-83. doi: 10.1083/jcb.201301053.

 

Development of a reentrant arrhythmia model in human pluripotent stem cell-derived cardiac cell sheets.

Kadota S, Minami I, Morone N, Heuser JE, Agladze K, Nakatsuji N.

Eur Heart J. 2013 Apr;34(15):1147-56. doi: 10.1093/eurheartj/ehs418.

 

A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions.

Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N.

Cell Rep. 2012 Nov 29;2(5):1448-60. doi: 10.1016/j.celrep.2012.09.015.

 

Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery.

Tanaka T, Takahashi K, Yamane M, Tomida S, Nakamura S, Oshima K, Niwa A, Nishikomori R, Kambe N, Hara H, Mitsuyama M, Morone N, Heuser JE, Yamamoto T, Watanabe A, Sato-Otsubo A, Ogawa S, Asaka I, Heike T, Yamanaka S, Nakahata T, Saito MK.

Blood. 2012 Aug 9;120(6):1299-308. doi: 10.1182/blood-2012-03-417881.

 

Utilization of photoinduced charge-separated state of donor-acceptor-linked molecules for regulation of cell membrane potential and ion transport.

Numata T, Murakami T, Kawashima F, Morone N, Heuser JE, Takano Y, Ohkubo K, Fukuzumi S, Mori Y, Imahori H.

J Am Chem Soc. 2012 Apr 11;134(14):6092-5. doi: 10.1021/ja3007275.

 

Membrane mechanisms for signal transduction: the coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes.

Kusumi A, Fujiwara TK, Morone N, Yoshida KJ, Chadda R, Xie M, Kasai RS, Suzuki KG.

Semin Cell Dev Biol. 2012 Apr;23(2):126-44. doi: 10.1016/j.semcdb.2012.01.018.

 

Cells respond to mechanical stress by rapid disassembly of caveolae.

Sinha B, Köster D, Ruez R, Gonnord P, Bastiani M, Abankwa D, Stan RV, Butler-Browne G, Vedie B, Johannes L, Morone N, Parton RG, Raposo G, Sens P, Lamaze C, Nassoy P.

Cell. 2011 Feb 4;144(3):402-13. doi: 10.1016/j.cell.2010.12.031.

 

Extended morphological processing: a practical method for automatic spot detection of biological markers from microscopic images.

Kimori Y, Baba N, Morone N.

BMC Bioinformatics. 2010 Jul 8;11:373. doi: 10.1186/1471-2105-11-373.

 

Freeze-etch electron tomography for the plasma membrane interface.

Morone N.

Methods Mol Biol. 2010;657:275-86. doi: 10.1007/978-1-60761-783-9_22.

Head of Electron Microscopy & Ultrastructural Pathology Facility

Contact Details

MRC Toxicology Unit
Hodgkin Building
Lancaster Road, Leicester
LE1 7HB

Telephone and Email

+44 (0)116 252 5609

Affiliations

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