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MRC Toxicology Unit

 

Biography

2015-Present Senior Investigator Scientist at MRC Toxicology Unit and visiting Professor at Kyoto University, Kyoto, Japan.

2010-2015 Senior Lecturer at iCeMS Kyoto University, Kyoto, Japan.

2005-2010 Section Chief at National Institute of Neuroscience, National Centre of Neurology and Psychiatry, Tokyo, Japan.

2004-2005 PI at Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan.

1999-2003 Postdoctoral at ERATO-JST/Nagoya University, Nagoya, Japan.

1999 PhD (Kyoto University, Japan) Synthetic & Biological Chemistry.

Research

Research Summary

Nobu’s research in the Unit is based on “EM-based nano-toxicology” for visual understanding the role of endocytosis at the plasma membrane and membrane recycling among the intracellular organelle compartments affected by toxins or drugs for mesothelioma in immune-oncology and neurogenerative diseases as well. Nobu’s EM covers a whole range of cellular tissues from the mouse brain to the signalling receptor-complexes by using both classic and advanced microscopy from deep-etch EM and cryo-EM (single particle analysis and tomography). Nobu’s future goal is to determine the atomic structure of crucial membrane machinery (caveolae with MSK) and chromatin complex in situ by the hybrid EM correlation, as well as to establish a lesion detection system under EM resolution by a template matching method toward tumour and neurogenerative diseases.

Publications

Key publications: 

Song Y, Dagil L, Fairall L, Robertson N, Wu M, Ragan TJ, Savva CG, Saleh A, Morone N, Kunze MBA, Jamieson AG, Cole PA, Hansen DF, Schwabe JWR. Mechanism of crosstalk between the LSD1 demethylase and HDAC1 deacetylase in the CoREST complex. Cell Rep. 2020 Feb 25;30(8):2699-2711.e8. doi: 10.1016/j.celrep.2020.01.091.

 

Kim H, Nobeyama T, Honda S, Yasuda K, Morone N, Murakami T. Membrane fusogenic high-density lipoprotein nanoparticles. Biochim Biophys Acta Biomembr. 2019 Oct 1;1861(10):183008. doi: 10.1016/j.bbamem.2019.06.007.

 

Robinson SW, Bourgognon JM, Spiers JG, Breda C, Campesan S, Butcher A, Mallucci GR, Dinsdale D, Morone N, Mistry R, Smith TM, Guerra-Martin M, Challiss RAJ, Giorgini F, Steinert JR. Nitric oxide-mediated posttranslational modifications control neurotransmitter release by modulating complexin farnesylation and enhancing its clamping ability. PLoS Biol. (2018) 16(4):e2003611. doi: 10.1371/journal.pbio.2003611.

 

Nishimura T, Morone N, Suetsugu S. Membrane re-modelling by BAR domain superfamily proteins via molecular and non-molecular factors. Biochem Soc Trans. (2018) 46(2):379-389. doi: 10.1042/BST20170322.

 

Li J, Minami I, Shiozaki M, Yu L, Yajima S, Miyagawa S, Shiba Y, Morone N, Fukushima S, Yoshioka M, Li S, Qiao J, Li X, Wang L, Kotera H, Nakatsuji N, Sawa Y, Chen Y, Liu L. Human pluripotent stem cell-derived cardiac tissue-like constructs for repairing the infarcted myocardium. Stem Cell Reports. (2017) 9(5):1546-1559. doi: 10.1016/j.stemcr.2017.09.007

 

Tachikawa M, Morone N, Senju Y, Sugiura T, Hanawa-Suetsugu K, Mochizuki A, Suetsugu S. Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension. Sci Rep. (2017) 7(1):7794. doi: 10.1038/s41598-017-08259-5.

 

Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T. Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency. Cell Metab. (2017) 25(5):1103-1117. doi: 10.1016/j.cmet.2017.04.017.

 

Kim H, Okamoto H, Felber AE, Polomska A, Morone N, Heuser JE, Leroux JC, Murakami T. Polymer-coated pH-responsive high-density lipoproteins. J Control Release. (2016) 228:132-140. doi: 10.1016/j.jconrel.2016.03.005.

 

Honda M, Minami I, Tooi N, Morone N, Nishioka H, Uemura K, Kinoshita A, Heuser JE, Nakatsuji N, Aiba K. The modeling of Alzheimer's disease by the overexpression of mutant Presenilin 1 in human embryonic stem cells. Biochem Biophys Res Commun. (2016) 469(3):587-92. doi: 10.1016/j.bbrc.2015.12.025.

 

Agostini M, Romeo F, Inoue S, Niklison-Chirou MV, Elia AJ, Dinsdale D, Morone N, Knight RA, Mak TW, Melino G. Metabolic reprogramming during neuronal differentiation. Cell Death Differ. (2016) doi: 10.1038/cdd.2016.36.

 

Fujiwara TK, Iwasawa K, Kalay Z, Tsunoyama TA, Watanabe Y, Umemura YM, Murakoshi H, Suzuki KG, Nemoto YL, Morone N, Kusumi A. Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane. Mol Biol Cell. (2016) ;27(7):1101-19. doi: 10.1091/mbc.E15-04-0186.

 

Sinha B, Köster D, Ruez R, Gonnord P, Bastiani M, Abankwa D, Stan RV, Butler-Browne G, Vedie B, Johannes L, Morone N, Parton RG, Raposo G, Sens P, Lamaze C, Nassoy P. Cells respond to mechanical stress by rapid disassembly of caveolae. Cell. (2011) 144(3):402-13.

 

Morone N, Nakada C, Umemura Y, Usukura J, Kusumi A. Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography. Methods Cell Biol. (2008) 88: 207-36.

 

Yao I, Takagi H, Ageta H, Kahyo T, Sato S, Hatanaka K, Fukuda Y, Chiba T, Morone N, Yuasa S, Inokuchi K, Ohtsuka T, Macgregor GR, Tanaka K, Setou M. SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Cell. (2007) 130:943-57.

 

Morone N, Fujiwara T, Murase K, Kasai SR, Ike H, Yuasa S, Usukura J, Kusumi A. Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. J Cell Biol. (2006) 174:851-62.

Head of Electron Microscopy & Ultrastructural Pathology Facility

Contact Details

MRC Toxicology Unit
Hodgkin Building
Lancaster Road, Leicester
LE1 7HB

Telephone and Email

+44 (0)116 252 5609

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