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The Flow Cytometry Facility has three analytical cytometers including one with plate-reading (HTS).

Instruments available:

  • BD Fortessa LSR (5 lasers, can detect up to 18 colours at once)
  • BD Fortessa LSR with high-throughput sampler, HTS (4 lasers, can detect up to 16 colours at once) – able to take 96-well plates for analysis of cells in suspension
  • BD FACS Canto II (2 lasers, can detect up to 6 fluorescent colours at once). It has a carrousel for automated acquisition

Examples of applications:

  • Immunophenotyping
  • Cell cycle
  • Proliferation assays
  • Apoptosis and cell viability assays

The analysers are user-operated but all users require at least one training session before they can be used independently. Training should be requested by submitting the flow cytometry training form (available on request or in the Unit’s Intranet) to cytometry@mrc-tox.cam.ac.uk.

Please contact us before you set up any experiment.We can advise and help you planning your experiments in addition to train you to use the instruments.

We welcome enquires regarding possible uses of flow cytometry in your research projects.