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Flow cytometry is a great technique for the analysis of multi parameters of all types of single cells and microparticles in suspension.  Through hydrodynamic focusing one cell at a time passes through a laser beam, and thousands of cells can be quickly examined.  Apart from analysing the intrinsic properties of cells, it examines fluorescence intensities expressed on the cell surface or intracellularly.

The Unit’s Flow Cytometry facility has 3 FACS analysers one FACS sorter. In addition to the maintenance of the instruments, assistance with experimental design and troubleshooting, advice on sample preparation and training on the instrument operation, we offer individualized assistance on the data analysis. It is strongly recommended that the planning of the experiment and the choice of dyes is discussed prior to ordering any antibodies/compounds to ensure they fit the instrument configurations. New users should contact Dr Lucia Piñon (lpp29@mrc-tox. cam.ac.uk) for training, and experimental set up advice.  Once users are trained, they can operate the FACS analysers by themselves, whilst the FACS sorter (BD FACS AriaII) is operated solely by the head of the facility (Dr Lucia Piñon P. Giraldez).

Instruments available:

BD FACSAria II (Room 03-0111) cell sorter and analyser which has five lasers (Blue (488 nm & 3PMTs), Yellow Green (561 nm& 5PMTs), Red (640 nm&3PMTs), UV (355 nm&2PMTs) and Violet (405 nm& 3PMTs)) and can detect up to 16 colours simultaneously. It has an aerosol management installed and able to perform four-way sorting as well as automatic cell deposition for sorting onto tubes, TC plates or slides. Sort rates are up to 20,000 cells per second.

BD Fortessa LSR (Room 02- 0118) 5 lasers, Blue (488nm &2PMTs), Red (640nm & 3PMTs), Violet (405nm &6PMTs) Yellow-Green (561nm & 5PMTs) and UV (355nm & 2PMTs)

BD Fortessa LSR with high throughput sampler (Room 03-0112) 4 lasers; Blue (488nm &2PMTs), Red (640nm & 3PMTs), Violet (405nm &6PMTs) Yellow-Green (561nm & 5PMTs)

BD FACS Canto II (Room 03-0112) 2 lasers; (Blue argon,488nm and Red HeNe, 640nm) capable of detecting up to six fluorescent signals (FITC, PE, PE-Cy5.5, PE-Cy7, APC and APC-Cy7)

Group members