We use freeze replication for studying plasma membranes, cytoskeletons, organelles and macromolecular complexes.
Methods:
1) Freeze-fracture.
2) Deep-etch.
3) SDS-FRIL (SDS-digested freeze fracture replica immunogold labelling).
4) DRIL (Deep-etch replica immunogold labelling.
Workflow:
Under construction.
Deep-etch images of the plasma membrane in tumour urinary bladders.
Image shows caveolae, clathrin-coated pits and an actin-based membrane skeleton on the cytoplasmic surface.