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The Advanced Light Imaging facility has a wide range of instruments and technologies to facilitate the Toxicology Unit’s Research. Comprehensive training and on-going support on all instruments and technologies within the facility is provided by Dr. Lucia Piñon P. Giraldez. There are also software packages for the processing and analysis of light-captured images. The facility is equipped with top of the range instruments including, 3 laser scanning confocal microscopes, a spinning disk confocal, two live imaging systems, a high content imaging system and a number of widefield microscopes.

 

Drosophila gastrointestinal tract

 

Instruments available and their location

 

Confocal Zeiss LSM 980 multiphoton inverted with AiryScan2 (Room 01-0111)

 

Confocal Zeiss LSM 880 inverted with Airyscan (Room 02—0120)

 

3i Spinning Disk confocal inverted microscope (with UV Ablate laser) (Room 03-0117)

 

Zeiss Axio Observer 7 with high-speed calcium imaging (Room 03-0117)

 

Laser microdissection Zeiss PALM Microbeam Microscope (Room 01-0111)

 

Confocal Zeiss LSM 510 with multiphoton (Room 03-0120)

 

Image analysis workstations with leading software packages

Zen Blue and Intellesis (Room 03-0113)

Imaris (Room 03-0113)

FlowJo in two analysis computers (Room 03-0113)

FCS Express (Room 02-0118)

 

Live Imaging

 IncuCyte (Sartorious) (Room 01- 0123)

xCELLigence (Room -1-0122)

 

High Content Screening and Analysis instrument -Cellomics -Thermo Arrayscan VTi from Thermo Scientific equipped with Studio Software. with a live imaging mode (temperature and CO2 levels controlled) (Room 03-0114)

It uses automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from adherent cell populations. HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. It is a powerful tool for research into cellular and systems biology and drug discovery. Cell and nuclear masks are used for automated demarcation, robust functional probes for cell health interrogation, a flexible assay workflow for automated processing, and ample fluorophore choice for easy multiplexing.

Some examples of the bioapplications that have been performed in the Unit are:

  1. Analysis of cell viability, cell count and transfection rate
  2. Proliferation and migration
  3. Apoptosis.
  4. Cell cycle and DNA repair.
  5. Cellular translocation
  6. Mitochondrial health.

                             

 

HepG2 cells (Mitochondria ATP5B)                 

Group members