Advanced Light Imaging

The Advanced Light Imaging Section provides a wide range of instruments and software for the acquisition, processing and analysis of light-captured images. The equipment is used by all Unit Research Groups together with external collaborators.

Within the Toxicology Unit, we have a range of instruments to capture images from a variety of experimental situations. The systems include three -scanning confocal microscopes, two of which are equipped with Mai Tai pulsing IR lasers for multi-photon imaging. Live cells can be imaged with an on-stage incubation system.

In addition, we have a new spinning-disk confocal incorporating a steerable UV ablation laser for DNA damage and other studies.

The Unit has a number of large wide-field microscope systems which are dedicated to time-lapse, calcium and live-cell imaging.

Support and user training is provided for all the instruments as well as help in analysing results using programs such as Volocity.

Mouse embryonic stem cell derived neuronal cells.

Courtesy of Dr Lia Panman, Toxicology Unit.

CHO M1 WT cells.

Courtesy of Dr Sophie Bradley, Toxicology Unit.

Stem cell derived dopaminergic neurons.

Courtesy of Dr Anthony Oosterveen, Toxicology Unit.

Selected Publications

Acute hyperbilirubinaemia induces presynaptic neurodegeneration at a central glutamatergic synapse.

Haustein MD, Read DJ, Steinert JR, Pilati N, Dinsdale D, Forsythe ID.

J Physiol. 2010 Dec 1;588(Pt 23):4683-93. Epub 2010 Oct 11.

Regulation of Kv channel expression and neuronal excitability in rat medial nucleus of the trapezoid body maintained in organotypic culture.

Tong H, Steinert JR, Robinson SW, Chernova T, Read DJ, Oliver DL, Forsythe ID.

J Physiol. 2010 May 1;588(Pt 9):1451-68. Epub 2010 Mar 8.

Advanced Light Imaging